mTq2-Calmodulin
(Plasmid #198200)

• Purpose: FRET donor: Calmodulin-2 tagged with N-terminal mTurquoise2
• Depositing Lab: Christian Wahl-Schott
• Publication: Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. doi: 10.1016/j.xpro.2023.102459.

Backbone

• Vector backbone: pcDNA3.1+
• Backbone size w/o insert (bp): 5335
• Total vector size (bp): 6505
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Calmodulin 2
• Alt name: Calm2
• Species: R. norvegicus (rat)
• Insert Size (bp): 444
• GenBank ID: NM_017326.3
• Entrez Gene: Calm2 (a.k.a. CaMII, Cam, Cam2, Camb)
• Tag / Fusion Protein:
  • mTurquoise2 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: GGCTAACTAGAGAACCCACTG
• 3′ sequencing primer: GGCAACTAGAAGGCACAGTC

Resource Information

• Supplemental Documents:
  • mTq2-Calmodulin.gbk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry