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Addgene

pNLF1-C_hCOL4A5-Nluc_E130X
(Plasmid #198117)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198117 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNLF1-C
  • Backbone manufacturer
    Promega
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    COL4A5
  • Species
    H. sapiens (human)
  • Mutation
    E130X
  • Entrez Gene
    COL4A5 (a.k.a. ASLN, ATS, ATS1, CA54)
  • Promoter CMV, beta-globin/IgG chimeric intron
  • Tag / Fusion Protein
    • Nluc (C terminal on insert)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNLF1-C_hCOL4A5-Nluc_E130X was a gift from Jeffrey Miner (Addgene plasmid # 198117 ; http://n2t.net/addgene:198117 ; RRID:Addgene_198117)

  • For your References section:

    NanoLuc reporters identify COL4A5 nonsense mutations susceptible to drug-induced stop codon readthrough. Omachi K, Kai H, Roberge M, Miner JH. iScience. 2022 Feb 8;25(3):103891. doi: 10.1016/j.isci.2022.103891. eCollection 2022 Mar 18. 10.1016/j.isci.2022.103891 PubMed 35243249
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