pCI-Puro_hCOL4A2-Myc-His-WPRE
(Plasmid
#198080)
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PurposeExpress hCOL4A2 in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 198080 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCI
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Backbone manufacturerPromega
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCOL4A2
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SpeciesH. sapiens (human)
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Entrez GeneCOL4A2 (a.k.a. BSVD2, ICH, POREN2)
- Promoter CMV, beta-globin/IgG chimeric intron
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Tag
/ Fusion Protein
- TEV-Myc-His (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCI-Puro_hCOL4A2-Myc-His-WPRE was a gift from Jeffrey Miner (Addgene plasmid # 198080 ; http://n2t.net/addgene:198080 ; RRID:Addgene_198080)