Ai-LV (CS-TRE-mOKS-PRE-Ubc-rtTA-I2G)
(Plasmid
#198058)
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PurposeGeneration of rodent iPS cells from somatic cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 198058 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
- Total vector size (bp) 15735
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemOct4, mKlf4, mSox2
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Alt namemOct4
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Alt namemKlf4
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Alt namemSox2
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SpeciesM. musculus (mouse)
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Insert Size (bp)3591
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Entrez GeneSox2 (a.k.a. Sox-2, lcc, ysb)
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Entrez GenePou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
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Entrez GeneKlf4 (a.k.a. EZF, Gklf, Zie)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ai-LV (CS-TRE-mOKS-PRE-Ubc-rtTA-I2G) was a gift from Hiromitsu Nakauchi (Addgene plasmid # 198058 ; http://n2t.net/addgene:198058 ; RRID:Addgene_198058) -
For your References section:
The generation and maintenance of rat induced pluripotent stem cells. Yamaguchi T, Hamanaka S, Nakauchi H. Methods Mol Biol. 2014;1210:143-50. doi: 10.1007/978-1-4939-1435-7_11. 10.1007/978-1-4939-1435-7_11 PubMed 25173166