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Addgene

pNIC-Bio2_SMAC(delta(55))_9His
(Plasmid #197976)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197976 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNIC-Bio2
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smac
  • Alt name
    Diablo
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    579
  • Mutation
    delta (1-55)
  • Entrez Gene
    DIABLO (a.k.a. DFNA64, SMAC)
  • Tag / Fusion Protein
    • 9His (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Electroporation recommended.

Please visit https://www.biorxiv.org/content/10.1101/2022.08.19.504532v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNIC-Bio2_SMAC(delta(55))_9His was a gift from Eric Fischer (Addgene plasmid # 197976 ; http://n2t.net/addgene:197976 ; RRID:Addgene_197976)

  • For your References section:

    Structures of BIRC6-client complexes provide a mechanism of SMAC-mediated release of caspases. Hunkeler M, Jin CY, Fischer ES. Science. 2023 Mar 17;379(6637):1105-1111. doi: 10.1126/science.ade5750. Epub 2023 Feb 9. 10.1126/science.ade5750 PubMed 36758104
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