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pLV-EF1a-AcGFP-P2A-hBRD4S
(Plasmid #197882)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197882 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVH-EF1a-GFP-P2A
  • Backbone size w/o insert (bp) 8317
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BRD4
  • Alt name
    BRD4S
  • Alt name
    HUNK1
  • Alt name
    MCAP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2169
  • GenBank ID
    NM_014299
  • Entrez Gene
    BRD4 (a.k.a. CAP, CDLS6, FSHRG4, HUNK1, HUNKI, MCAP)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer AAGTACAACCCTCCTGACCA
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-EF1a-AcGFP-P2A-hBRD4S was a gift from Kejin Hu (Addgene plasmid # 197882 ; http://n2t.net/addgene:197882 ; RRID:Addgene_197882)

  • For your References section:

    Attenuating iPSC reprogramming stress with dominant-negative BET peptides. Hossain ME, Cevallos RR, Zhang R, Hu K. iScience. 2022 Dec 28;26(1):105889. doi: 10.1016/j.isci.2022.105889. eCollection 2023 Jan 20. 10.1016/j.isci.2022.105889 PubMed 36691621
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