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Addgene

AAV-PR rep/cap
(Plasmid #197565)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197565 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAR9
  • Total vector size (bp) 7486
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    AAV Rep genes
  • Species
    parvovirus
  • Insert Size (bp)
    1863

Gene/Insert 2

  • Gene/Insert name
    AAV9 VP1 Cap gene with PR insert
  • Species
    parvovirus
  • Insert Size (bp)
    2229
  • Mutation
    contains 7mer insert PRPPSTH between amino acids 588 and 589 of AAV9 VP1 protein

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-PR rep/cap was a gift from Casey Maguire (Addgene plasmid # 197565 ; http://n2t.net/addgene:197565 ; RRID:Addgene_197565)

  • For your References section:

    An engineered AAV capsid mediates efficient transduction of pericytes and smooth muscle cells of the brain vasculature. Ramirez SH, Hale J, McCarthy S, Cardenas CL, Dona K, Hanlon KS, Hudry E, De La Cruz D, Ng C, Das S, Nguyen DM, Nammour J, Bennett RE, Andrews A, Musolino PL, Maguire CA. Hum Gene Ther. 2023 Jun 27. doi: 10.1089/hum.2022.211. 10.1089/hum.2022.211 PubMed 37376759
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