pAAV-hChATp-tdTomato
(Plasmid #197564)

• Purpose: Expresses tdTomato under a human choline acetyltransferase (hChAT) promoter fragment
• Depositing Lab: Casey Maguire
• Publication: Santoscoy et al Mol Ther Methods Clin Dev. 2023 May 9;29:532-540. doi: 10.1016/j.omtm.2023.05.001. eCollection 2023 Jun 8.

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Backbone

• Vector backbone: pAAV-CAG-tdTomato Addgene 59462
• Total vector size (bp): 6278
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: This plasmid contains AAV ITR sequences so care must be taken to screen for intact ITRs at each step of plasmid production/cloning.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: tdTomato
• Species: Discosoma sp.
• Insert Size (bp): 1431
• Promoter: human ChAT promoter

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: NA

Resource Information

• Supplemental Documents:
  • pAAV-pChAT-tdTomato.gb
• A portion of this plasmid was derived from a plasmid made by: We received the original plasmid from Addgene 59462

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry