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Addgene

pAAV-hChATp-tdTomato
(Plasmid #197564)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197564 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-CAG-tdTomato Addgene 59462
  • Total vector size (bp) 6278
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    This plasmid contains AAV ITR sequences so care must be taken to screen for intact ITRs at each step of plasmid production/cloning.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    tdTomato
  • Species
    Discosoma sp.
  • Insert Size (bp)
    1431
  • Promoter human ChAT promoter

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    We received the original plasmid from Addgene 59462

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hChATp-tdTomato was a gift from Casey Maguire (Addgene plasmid # 197564 ; http://n2t.net/addgene:197564 ; RRID:Addgene_197564)

  • For your References section:

    An AAV capsid increases transduction of striatum and a ChAT promoter allows selective cholinergic neuron transduction. Santoscoy MC, Espinoza P, De La Cruz D, Mahamdeh M, Starr JR, Patel N, Maguire CA. Mol Ther Methods Clin Dev. 2023 May 9;29:532-540. doi: 10.1016/j.omtm.2023.05.001. eCollection 2023 Jun 8. 10.1016/j.omtm.2023.05.001 PubMed 37359416
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