pSpCas9(BB)-2A-mCherry
(Plasmid #197420)

• Purpose: Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA
• Depositing Lab: Torsten Wittmann
• Publication: Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.7554/eLife.84143.

Backbone

• Vector backbone: PX458
• Backbone size w/o insert (bp): 4239
• Total vector size (bp): 9300
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cas9
• Species: S. pyogenes
• Insert Size (bp): 5061
• GenBank ID:
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Resource Information

• Supplemental Documents:
  • pSpCas9_BB_2A-mCherry.gbk
• A portion of this plasmid was derived from a plasmid made by: Addgene #48138

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.09.22.509085v1 for bioRxiv preprint.