pSD5
(Plasmid #197289)

• Purpose: Contains a seamless deletion cassette for the replacement of ORF2230 in D. radiodurans
• Depositing Lab: Bogumil Karas
• Publication: Brumwell et al Biodes Res. 2023 Mar 15;5:0009. doi: 10.34133/bdr.0009. eCollection 2023.

Backbone

• Vector backbone: pCC1BAC
• Backbone size w/o insert (bp): 17996
• Total vector size (bp): 19319
• Modifications to backbone: Addition of CEN-ARS-HIS sequences for replication and selection in S. cerevisiae
• Vector type: Synthetic Biology ; Bacterial and Yeast Cloning
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): E. coli Epi300
• Growth instructions: Plasmid can be induced to high copy with arabinose.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: NptII
• Alt name: Neomycin resistance gene
• Insert Size (bp): 1323

Resource Information

• Supplemental Documents:
  • pdeino45_2230_trec_nm_.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.09.21.508766v1 for bioRxiv preprint.