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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 19715 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneCAGEN
- Backbone size w/o insert (bp) 4801
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameengrailed
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SpeciesD. melanogaster (fly)
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Insert Size (bp)953
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MutationDrosophila engrailed repressor domain
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Entrez Geneen (a.k.a. Dmel_CG9015, 153867_at, Apa, CG9015, Dmel\CG9015, EN, En, EnR, Eng, Engr, Engrailed, Engrailed/Invected, Es, V, en1, spa2, spt)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (destroyed during cloning)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CAG-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CAG-EnR was a gift from Connie Cepko (Addgene plasmid # 19715 ; http://n2t.net/addgene:19715 ; RRID:Addgene_19715) -
For your References section:
A core paired-type and POU homeodomain-containing transcription factor program drives retinal bipolar cell gene expression. Kim DS, Matsuda T, Cepko CL. J Neurosci. 2008 Jul 30. 28(31):7748-64. 10.1523/JNEUROSCI.0397-08.2008 PubMed 18667607
Map uploaded by the depositor.
