pGC235
(Plasmid #19683)

• Depositing Lab: Jane Hubbard
• Publication: Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.

Backbone

• Vector backbone: pDONR 221
• Vector type: Gateway entry clone

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Plim-7
• Species: C. elegans (nematode)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid has been found to be prone to multimerization. Plasmid multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.