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Addgene

pCW-Tet-Cas9-BFP-PGK-Blast
(Plasmid #196720)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196720 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCW-Cas9-Blast (#83481)
  • Backbone manufacturer
    Eric Lander, David Sabatini
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers
    Blasticidin ; BFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cas9-T2A-TagBFP
  • Alt name
    Blast-T2A-rtTA
  • Species
    S. pyogenes
  • Promoter TRE, PGK
  • Tags / Fusion Proteins
    • 3xFLAG-SV40NLS (N terminal on insert)
    • Nucleoplasmin NLS (C terminal on insert)

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: Doxycycline creates autofluorescence in the BFP channel in flow cytometry. Needs to be washed out before FACS

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW-Tet-Cas9-BFP-PGK-Blast was a gift from Bernhard Schmierer (Addgene plasmid # 196720 ; http://n2t.net/addgene:196720 ; RRID:Addgene_196720)
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