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pGEX-4T-1-ε ear
(Plasmid #196670)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196670 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T-1
  • Total vector size (bp) 4969
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AP-4 ε ear (840-1137)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    902
  • Mutation
    silent mutations in codons 905, 1129 and 1137
  • Entrez Gene
    AP4E1 (a.k.a. CPSQ4, SPG51, STUT1)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GST forward
  • 3′ sequencing primer GST reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T-1-ε ear was a gift from Juan Bonifacino (Addgene plasmid # 196670 ; http://n2t.net/addgene:196670 ; RRID:Addgene_196670)

  • For your References section:

    Bivalent Motif-Ear Interactions Mediate the Association of the Accessory Protein Tepsin with the AP-4 Adaptor Complex. Mattera R, Guardia CM, Sidhu SS, Bonifacino JS. J Biol Chem. 2015 Dec 25;290(52):30736-49. doi: 10.1074/jbc.M115.683409. Epub 2015 Nov 5. 10.1074/jbc.M115.683409 PubMed 26542808
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