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Addgene

pGBT9-tepsin
(Plasmid #196647)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196647 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGBT9
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7092
  • Vector type
    Yeast Expression ; yeast two-hybrid vector
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tepsin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1578
  • Mutation
    silent substitution in codon 199 to eliminate internal EcoRI site
  • Entrez Gene
    TEPSIN (a.k.a. C17orf56, ENTHD2)
  • Tag / Fusion Protein
    • GAL4-DNA binding domain fragment (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer GAL4 DNA binding domain forward
  • 3′ sequencing primer internal tepsin primer sequence: 5' CAG GAG TTG AGC TTG GTG AGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBT9-tepsin was a gift from Juan Bonifacino (Addgene plasmid # 196647 ; http://n2t.net/addgene:196647 ; RRID:Addgene_196647)

  • For your References section:

    Bivalent Motif-Ear Interactions Mediate the Association of the Accessory Protein Tepsin with the AP-4 Adaptor Complex. Mattera R, Guardia CM, Sidhu SS, Bonifacino JS. J Biol Chem. 2015 Dec 25;290(52):30736-49. doi: 10.1074/jbc.M115.683409. Epub 2015 Nov 5. 10.1074/jbc.M115.683409 PubMed 26542808
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