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Addgene

pcDNA 3.1-TSF-ε
(Plasmid #196583)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196583 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA 3.1
  • Backbone size w/o insert (bp) 5430
  • Total vector size (bp) 8980
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AP-4 ε subunit
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3543
  • Entrez Gene
    AP4E1 (a.k.a. CPSQ4, SPG51, STUT1)
  • Tag / Fusion Protein
    • two Strep/one FLAG (TSF) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer bGH reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA 3.1-TSF-ε was a gift from Juan Bonifacino (Addgene plasmid # 196583 ; http://n2t.net/addgene:196583 ; RRID:Addgene_196583)

  • For your References section:

    Bivalent Motif-Ear Interactions Mediate the Association of the Accessory Protein Tepsin with the AP-4 Adaptor Complex. Mattera R, Guardia CM, Sidhu SS, Bonifacino JS. J Biol Chem. 2015 Dec 25;290(52):30736-49. doi: 10.1074/jbc.M115.683409. Epub 2015 Nov 5. 10.1074/jbc.M115.683409 PubMed 26542808
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