AAV-CAGGS-mNAGA
(Plasmid #196448)

• Purpose: Expresses modified α-N-acetylgalactosaminidase in mammalian cells
• Depositing Lab: Yuichiro Miyaoka
• Publication: Nakajima et al Cell Transplant. 2023 Jan-Dec;32:9636897231173734. doi: 10.1177/09636897231173734.

Backbone

• Vector backbone: AAV-CAGGS-EGFP
• Backbone manufacturer: Rudolf Jaenisch Lab
• Backbone size w/o insert (bp): 9102
• Total vector size (bp): 10380
• Modifications to backbone: Replaced the EGFP cDNA of AAV-CAGGS-EGFP (Addgene #22212) with mNAGA cDNA.
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: modified alpha-N-acetylgalactosaminidase
• Alt name: modified NAGA
• Alt name: Mod NAGA
• Alt name: mNAGA
• Species: H. sapiens (human)
• Insert Size (bp): 1278
• Entrez Gene: NAGA (a.k.a. D22S674, GALB)
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: MluI (not destroyed)
• 5′ sequencing primer: 5'-TTCGGCTTCTGGCGTGTGAC-3'
• 3′ sequencing primer: 5'-CAGGAAACAGCTATGACCATG-3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.01.03.522057v1 for bioRxiv preprint.