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pET22b-T5-sfGFP*- MmH-MH4R
(Plasmid #196409)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196409 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET
  • Backbone size w/o insert (bp) 5130
  • Total vector size (bp) 9130
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    sfGFP with TAG at 151 position

Gene/Insert 2

  • Gene/Insert name
    Tyrosine Hydroxylase
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Th

Gene/Insert 3

  • Gene/Insert name
    dihydropterine reductase from human
  • Species
    H. sapiens (human)
  • Entrez Gene
    QDPR (a.k.a. DHPR, HDHPR, PKU2, SDR33C1)

Gene/Insert 4

  • Gene/Insert name
    pterin-4a-carbinolamine dehydratase from human
  • Species
    H. sapiens (human)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET22b-T5-sfGFP*- MmH-MH4R was a gift from Han Xiao (Addgene plasmid # 196409 ; http://n2t.net/addgene:196409 ; RRID:Addgene_196409)

  • For your References section:

    Biosynthesis and Genetic Incorporation of 3,4-Dihydroxy-L-Phenylalanine into Proteins in Escherichia coli. Chen Y, Loredo A, Chung A, Zhang M, Liu R, Xiao H. J Mol Biol. 2021 Dec 20:167412. doi: 10.1016/j.jmb.2021.167412. 10.1016/j.jmb.2021.167412 PubMed 34942167
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