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Addgene

pGC320
(Plasmid #19639)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19639 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGC246
  • Vector type
    Worm Expression ; FLP/FRT, destination vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Growth instructions
    DB3.1 cells
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gateway(R4-R3)<iA-4xNLS-mCherry::let-858(3’)<iA-Wormgate::let-858(3’)
  • Species
    C. elegans (nematode), S. cerevisiae (budding yeast); Discosoma sp.
  • Tag / Fusion Protein
    • 4xNLS (N terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Sequence mismatches between Addgene sequence and depositor sequence do not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGC320 was a gift from Jane Hubbard (Addgene plasmid # 19639 ; http://n2t.net/addgene:19639 ; RRID:Addgene_19639)

  • For your References section:

    A "FLP-Out" system for controlled gene expression in Caenorhabditis elegans. Voutev R, Hubbard EJ. Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274. 10.1534/genetics.108.090274 PubMed 18723890
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