pGC92
(Plasmid
#19615)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 19615 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript SK+
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Vector typecarries FLP recombinase cDNA
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFLP
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SpeciesS. cerevisiae (budding yeast)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7, M13F
- 3′ sequencing primer T3, M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGC92 was a gift from Jane Hubbard (Addgene plasmid # 19615 ; http://n2t.net/addgene:19615 ; RRID:Addgene_19615) -
For your References section:
A "FLP-Out" system for controlled gene expression in Caenorhabditis elegans. Voutev R, Hubbard EJ. Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274. 10.1534/genetics.108.090274 PubMed 18723890
