pGC379
(Plasmid
#19647)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 19647 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGC305
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Vector typeWorm Expression ; FLP/FRT
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name<*-GFP::let-858(3’)
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SpeciesC. elegans (nematode), S. cerevisiae (budding yeast); A. victoria
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGC379 was a gift from Jane Hubbard (Addgene plasmid # 19647 ; http://n2t.net/addgene:19647 ; RRID:Addgene_19647) -
For your References section:
A "FLP-Out" system for controlled gene expression in Caenorhabditis elegans. Voutev R, Hubbard EJ. Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274. 10.1534/genetics.108.090274 PubMed 18723890
