pFSynW Substance P-pHluorin IRES mRuby3
(Plasmid #195699)

• Purpose: Lentiviral plasmid encoding first 68 residues of mouse preprotachykinin with a C-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter
• Depositing Lab: Edwin Chapman
• Publication: Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491. doi: 10.1523/JNEUROSCI.1857-22.2023. Epub 2023 Feb 2.

Backbone

• Vector backbone: FUGW
• Backbone manufacturer: David Baltimore (Addgene plasmid # 14883)
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tac1
• Species: M. musculus (mouse)
• Entrez Gene: Tac1 (a.k.a. 4930528L02Rik, NK-1, NK1, Nkna, PPT-A, PPTA, SP)
• Tag / Fusion Protein:
  • pHluorin (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pFSynW Substance P-pHluorin IRES mRuby3.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Only the first 68 amino acids of Tac1 are in this construct, followed by a flexible GS(GSS)4 linker, followed by superecliptic pHluorin; bicistronic mRuby3 expression.

Please visit https://www.biorxiv.org/content/10.1101/2022.04.18.488681v2 for bioRxiv preprint.