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Addgene

Preprotachykinin A
(Plasmid #130664)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 130664 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pFusionRed-N
  • Backbone manufacturer
    Evrogen
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Preprotachykinin A
  • Alt name
    prototachykinin-1
  • Alt name
    TAC1 (tachykinin precursor 1)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    387
  • Entrez Gene
    TAC1 (a.k.a. Hs.2563, NK2, NKNA, NPK, TAC2)
  • Promoter CMV
  • Tag / Fusion Protein
    • Fusion Red (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

brain cDNA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Preprotachykinin A was a gift from Michael Kressel (Addgene plasmid # 130664 ; http://n2t.net/addgene:130664 ; RRID:Addgene_130664)