Preprotachykinin A
(Plasmid
#130664)
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PurposeExpresses human Preprotachykinin A in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 130664 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFusionRed-N
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Backbone manufacturerEvrogen
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePreprotachykinin A
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Alt nameprototachykinin-1
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Alt nameTAC1 (tachykinin precursor 1)
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SpeciesH. sapiens (human)
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Insert Size (bp)387
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Entrez GeneTAC1 (a.k.a. Hs.2563, NK2, NKNA, NPK, TAC2)
- Promoter CMV
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Tag
/ Fusion Protein
- Fusion Red (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
brain cDNA
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Preprotachykinin A was a gift from Michael Kressel (Addgene plasmid # 130664 ; http://n2t.net/addgene:130664 ; RRID:Addgene_130664)