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Addgene

pCHA1.1-Rap2a-V5-Hygro
(Plasmid #195510)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195510 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCHA1.1
  • Backbone size w/o insert (bp) 7944
  • Total vector size (bp) 8982
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hygromycin
  • Species
    E. Coli
  • Insert Size (bp)
    1038
  • Promoter hPGK

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ttccgcattctgcaagcctc
  • 3′ sequencing primer ggctaagatctacagctgcc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCHA1.1-Rap2a-V5-Hygro was a gift from Joanna Wysocka (Addgene plasmid # 195510 ; http://n2t.net/addgene:195510 ; RRID:Addgene_195510)

  • For your References section:

    A genome-wide genetic screen uncovers determinants of human pigmentation. Bajpai VK, Swigut T, Mohammed J, Naqvi S, Arreola M, Tycko J, Kim TC, Pritchard JK, Bassik MC, Wysocka J. Science. 2023 Aug 11;381(6658):eade6289. doi: 10.1126/science.ade6289. Epub 2023 Aug 11. 10.1126/science.ade6289 PubMed 37561850
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