Pb-TRE3G-TYR-HygR
(Plasmid
#195508)
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Purposeinducible Piggybac vector expressing human TYR CDS with hygromycin selection
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195508 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepiggyBac
- Backbone size w/o insert (bp) 8017
- Total vector size (bp) 9705
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Vector typeMammalian Expression ; piggyBac
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTYR
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SpeciesH. sapiens (human)
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Insert Size (bp)1688
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Entrez GeneTYR (a.k.a. ATN, CMM8, OCA1, OCA1A, OCAIA, SHEP3)
- Promoter TRE
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer cacccgtgcgttttattctg
- 3′ sequencing primer atcgcctggagcaattccac (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Pb-TRE3G-TYR-HygR was a gift from Joanna Wysocka (Addgene plasmid # 195508 ; http://n2t.net/addgene:195508 ; RRID:Addgene_195508) -
For your References section:
A genome-wide genetic screen uncovers determinants of human pigmentation. Bajpai VK, Swigut T, Mohammed J, Naqvi S, Arreola M, Tycko J, Kim TC, Pritchard JK, Bassik MC, Wysocka J. Science. 2023 Aug 11;381(6658):eade6289. doi: 10.1126/science.ade6289. Epub 2023 Aug 11. 10.1126/science.ade6289 PubMed 37561850