Skip to main content
Addgene

MscL(G22S)61-cpGFP-ERexp
(Plasmid #195311)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195311 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    Puro
  • Backbone size w/o insert (bp) 6130
  • Total vector size (bp) 7282
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MscL(G22S)61-cpGFP-ERexp
  • Species
    Synthetic; E. coli
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ATGAGCATTATTAAAGAATTTCGCG
  • 3′ sequencing primer AACTTCATTTTCATAGCAAAAAGAGCG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MscL(G22S)61-cpGFP-ERexp was a gift from Allen Liu (Addgene plasmid # 195311 ; http://n2t.net/addgene:195311 ; RRID:Addgene_195311)

  • For your References section:

    Mechanosensitive Channel-Based Optical Membrane Tension Reporter. Hsu YY, Resto Irizarry AM, Fu J, Liu AP. ACS Sens. 2023 Jan 27;8(1):12-18. doi: 10.1021/acssensors.2c01921. Epub 2023 Jan 6. 10.1021/acssensors.2c01921 PubMed 36608338
Related items:
Commonly requested with: