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Addgene

GFP-MLC2 WT
(Plasmid #195235)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195235 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MLC2
  • Species
    H. sapiens (human)
  • Entrez Gene
    MYL2 (a.k.a. CMH10, MFM12, MLC-2, MLC-2s/v, MLC-2v, MLC2)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-MLC2 WT was a gift from David Bryant & Keith Mostov (Addgene plasmid # 195235 ; http://n2t.net/addgene:195235 ; RRID:Addgene_195235)

  • For your References section:

    Involvement of RhoA, ROCK I and myosin II in inverted orientation of epithelial polarity. Yu W, Shewan AM, Brakeman P, Eastburn DJ, Datta A, Bryant DM, Fan QW, Weiss WA, Zegers MM, Mostov KE. EMBO Rep. 2008 Sep;9(9):923-9. doi: 10.1038/embor.2008.135. Epub 2008 Jul 25. 10.1038/embor.2008.135 PubMed 18660750
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