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Addgene

GFP-PKCA D463N
(Plasmid #195203)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195203 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRKCA
  • Species
    H. sapiens (human)
  • Mutation
    D463N
  • Entrez Gene
    PRKCA (a.k.a. AAG6, PKC-alpha, PKCA, PKCI+/-, PKCalpha, PRKACA)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-PKCA D463N was a gift from David Bryant & Peter Parker (Addgene plasmid # 195203 ; http://n2t.net/addgene:195203 ; RRID:Addgene_195203)

  • For your References section:

    PKC maturation is promoted by nucleotide pocket occupation independently of intrinsic kinase activity. Cameron AJ, Escribano C, Saurin AT, Kostelecky B, Parker PJ. Nat Struct Mol Biol. 2009 Jun;16(6):624-30. doi: 10.1038/nsmb.1606. Epub 2009 May 24. 10.1038/nsmb.1606 PubMed 19465915
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