EF1a_Puro_Telo_v2
(Plasmid #195139)

• Purpose: ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction
• Depositing Lab: Jason Sheltzer
• Publication: Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.1126/science.adg4521.

Backbone

• Vector backbone: TelCas_ATmap
• Vector type: Mammalian Expression
• Selectable markers: mCherry

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Telomere
• Species: H. sapiens (human)
• Entrez Gene: RTEL1 (a.k.a. C20orf41, DKCA4, DKCB5, NHL, PFBMFT3, RTEL)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • EF1a_Puro_Telo_v2.gb
• A portion of this plasmid was derived from a plasmid made by: The telomere seed sequence was a generous gift from Alison Taylor.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.01.09.523344 for bioRxiv preprint.