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Addgene

GFP-Sharpin S146A
(Plasmid #195096)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195096 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SHARPIN
  • Species
    H. sapiens (human)
  • Mutation
    S146A
  • Entrez Gene
    SHARPIN (a.k.a. SIPL1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-Sharpin S146A was a gift from Jukka Westermarck (Addgene plasmid # 195096 ; http://n2t.net/addgene:195096 ; RRID:Addgene_195096)

  • For your References section:

    SHARPIN S146 phosphorylation mediates ARP2/3 interaction, cancer cell invasion and metastasis. Butt U, Khan MH, Pouwels J, Westermarck J. J Cell Sci. 2022 Oct 15;135(20):jcs260627. doi: 10.1242/jcs.260627. Epub 2022 Oct 13. 10.1242/jcs.260627 PubMed 36148554
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