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Addgene

pAAV-hSOX9-dTAG-mNeonGreen-V5
(Plasmid #194971)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194971 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV helper vector
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 6984
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SRY-box transcription factor 9
  • Alt name
    SOX9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2894
  • Entrez Gene
    SOX9 (a.k.a. CMD1, CMPD1, SRA1, SRXX2, SRXY10)
  • Tag / Fusion Protein
    • dTAG-mNeonGreen-V5 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSOX9-dTAG-mNeonGreen-V5 was a gift from Joanna Wysocka (Addgene plasmid # 194971 ; http://n2t.net/addgene:194971 ; RRID:Addgene_194971)

  • For your References section:

    Precise modulation of transcription factor levels identifies features underlying dosage sensitivity. Naqvi S, Kim S, Hoskens H, Matthews HS, Spritz RA, Klein OD, Hallgrimsson B, Swigut T, Claes P, Pritchard JK, Wysocka J. Nat Genet. 2023 May;55(5):841-851. doi: 10.1038/s41588-023-01366-2. Epub 2023 Apr 6. 10.1038/s41588-023-01366-2 PubMed 37024583
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