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Addgene

Cas12j-8-puro
(Plasmid #194966)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194966 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV2 ITR vector
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cas12j-8-puro was a gift from Yongming Wang (Addgene plasmid # 194966 ; http://n2t.net/addgene:194966 ; RRID:Addgene_194966)

  • For your References section:

    A highly specific CRISPR-Cas12j nuclease enables allele-specific genome editing. Wang Y, Qi T, Liu J, Yang Y, Wang Z, Wang Y, Wang T, Li M, Li M, Lu D, Chang ACY, Yang L, Gao S, Wang Y, Lan F. Sci Adv. 2023 Feb 10;9(6):eabo6405. doi: 10.1126/sciadv.abo6405. Epub 2023 Feb 10. 10.1126/sciadv.abo6405 PubMed 36763662
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