LV anti-FITC-LaG16-Y186F/Y229A-2Xmutant-T2A-BFP
(Plasmid
#194934)
-
PurposeLV construct encoding Y186F/Y229A double mutant sNRR-based SynNotch receptor
-
Depositing Lab
-
Sequence Information
-
Sequences (1) — Accept Affinity Reagent Sequence Policy
-
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 194934 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonen/a
-
Vector typeLentiviral, Synthetic Biology
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameantiFITC-LaG16-sNRR(Y186F/Y229A)-SynNotch-Gal4-VP64-T2A-BFP
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
LV anti-FITC-LaG16-Y186F/Y229A-2Xmutant-T2A-BFP was a gift from John Ngo (Addgene plasmid # 194934 ; http://n2t.net/addgene:194934 ; RRID:Addgene_194934) -
For your References section:
Tension-tuned receptors for synthetic mechanotransduction and intercellular force detection. Sloas DC, Tran JC, Marzilli AM, Ngo JT. Nat Biotechnol. 2023 Jan 16. doi: 10.1038/s41587-022-01638-y. 10.1038/s41587-022-01638-y PubMed 36646932