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pET28a-6xHis-MBP-TEV site-TadA8.20
(Plasmid #194702)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194702 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5201
  • Total vector size (bp) 6961
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TadA8.20
  • Species
    Synthetic
  • Insert Size (bp)
    501
  • Mutation
    mutations in TadA are described in the paper
  • GenBank ID
  • Promoter T7 promoter
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • MBP (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer agcatguctgaggtggagttttcccacg
  • 3′ sequencing primer atgctcgagutagtcggtggagctctgggc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-6xHis-MBP-TEV site-TadA8.20 was a gift from Weixin Tang (Addgene plasmid # 194702 ; http://n2t.net/addgene:194702 ; RRID:Addgene_194702)

  • For your References section:

    Transcriptome-wide profiling and quantification of N(6)-methyladenosine by enzyme-assisted adenosine deamination. Xiao YL, Liu S, Ge R, Wu Y, He C, Chen M, Tang W. Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01587-6. 10.1038/s41587-022-01587-6 PubMed 36593412
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