pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt
(Plasmid #194519)

• Purpose: Dual fluorescence reporter for studying protein degradation
• Depositing Lab: Tommer Ravid
• Publication: Mashahreh et al Nat Commun. 2022 Dec 8;13(1):7588. doi: 10.1038/s41467-022-35298-y.

Backbone

• Vector backbone: pRS315
• Backbone manufacturer: Academic publication
• Backbone size w/o insert (bp): 6018
• Total vector size (bp): 8907
• Modifications to backbone: ADH700 promoter, yeCherry, p150 IRES, Pca I/BamH I cloning site yeGFP, Xma I/Nhe I cloning site, Stop codon, Cyc1 terminator
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TIF4631 leader sequence
• Alt name: p150
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 470
• GenBank ID: 853071

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The insert was cloned by Edwards, S. R. & Wandless, T. J. and was extracted from Addgene deposition #24378

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry