GFP-myrlysin/BORCS5
(Plasmid
#194332)
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PurposeExpresses myrlysin in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 194332 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneGFP-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBORCS5
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Alt namemyrlysin, LOH12CR1
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SpeciesH. sapiens (human)
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Insert Size (bp)588
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Entrez GeneBORCS5 (a.k.a. LOH12CR1, LOH1CR12)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer GFP-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-myrlysin/BORCS5 was a gift from Juan Bonifacino (Addgene plasmid # 194332 ; http://n2t.net/addgene:194332 ; RRID:Addgene_194332) -
For your References section:
BORC, a multisubunit complex that regulates lysosome positioning. Pu J, Schindler C, Jia R, Jarnik M, Backlund P, Bonifacino JS. Dev Cell. 2015 Apr 20;33(2):176-88. doi: 10.1016/j.devcel.2015.02.011. 10.1016/j.devcel.2015.02.011 PubMed 25898167
