pcDNA3.1(+)-myc-OGA(GS-544)C181-D174N
(Plasmid
#193999)
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Purposeplasmid encoding myc-tagged OGA(GS-544)C181-D174N
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 193999 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5300
- Total vector size (bp) 8367
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemyc-OGA(GS-544)C181-D174N
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SpeciesH. sapiens (human)
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Tag
/ Fusion Protein
- myc (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(+)-myc-OGA(GS-544)C181-D174N was a gift from Christina Woo (Addgene plasmid # 193999 ; http://n2t.net/addgene:193999 ; RRID:Addgene_193999) -
For your References section:
Small Molecule-Activated O-GlcNAcase for Spatiotemporal Removal of O-GlcNAc in Live Cells. Ge Y, Lu H, Yang B, Woo CM. ACS Chem Biol. 2023 Jan 4. doi: 10.1021/acschembio.2c00894. 10.1021/acschembio.2c00894 PubMed 36598936