GreenT-EC.c - Display - CyRFP1
(Plasmid #193945)

• Purpose: Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface
• Depositing Lab: Oliver Griesbeck
• Publication: Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi: 10.1038/s41467-023-41928-w.

Backbone

• Vector backbone: pcDNA 3.1
• Backbone size w/o insert (bp): 5630
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GreenT-EC.c
• Species: Branchiostoma lanceolatum / Opsanus tau
• Insert Size (bp): 1998
• Tags / Fusion Proteins:
  • Igk_secretion - HA-Flag (N terminal on insert)
  • PDGFR (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: TAATACGACTCACTATAG
• 3′ sequencing primer: GAGGCTGATCAGCGGGTTTAAACGGGCC

Resource Information

• Supplemental Documents:
  • GreenT-EC.c - Display - CyRFP1 sequence file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry