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Addgene

GreenT-EC.b - Display - CyRFP1
(Plasmid #193944)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193944 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA 3.1
  • Backbone size w/o insert (bp) 5360
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GreenT-EC.b
  • Species
    Branchiostoma lanceolatum / Opsanus tau
  • Insert Size (bp)
    1998
  • Tags / Fusion Proteins
    • Igk_secretion - HA-Flag (N terminal on insert)
    • PDGFR (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TAATACGACTCACTATAG
  • 3′ sequencing primer GAGGCTGATCAGCGGGTTTAAACGGGCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GreenT-EC.b - Display - CyRFP1 was a gift from Oliver Griesbeck (Addgene plasmid # 193944 ; http://n2t.net/addgene:193944 ; RRID:Addgene_193944)

  • For your References section:

    Fluorescent sensors for imaging of interstitial calcium. Valiente-Gabioud AA, Garteizgogeascoa Suner I, Idziak A, Fabritius A, Basquin J, Angibaud J, Nagerl UV, Singh SP, Griesbeck O. Nat Commun. 2023 Oct 5;14(1):6220. doi: 10.1038/s41467-023-41928-w. 10.1038/s41467-023-41928-w PubMed 37798285
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