ChlorON-1
(Plasmid
#193477)
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PurposeTurn-on fluorescent sensor for chloride
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 193477 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDNA3.1(+)-N-6His
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Backbone manufacturerGenscript
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameChlorON-1
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SpeciesSynthetic
- Promoter CMV
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Tag
/ Fusion Protein
- 6xHis (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ChlorON-1 was a gift from Sheel Dodani (Addgene plasmid # 193477 ; http://n2t.net/addgene:193477 ; RRID:Addgene_193477) -
For your References section:
Engineering the ChlorON Series: Turn-On Fluorescent Protein Sensors for Imaging Labile Chloride in Living Cells. Tutol JN, Ong WSY, Phelps SM, Peng W, Goenawan H, Dodani SC. ACS Cent Sci. 2023 Dec 18;10(1):77-86. doi: 10.1021/acscentsci.3c01088. eCollection 2024 Jan 24. 10.1021/acscentsci.3c01088 PubMed 38292617