pMXs-Sox2-17
(Plasmid #193353)

• Purpose: Retroviral vector expressing mouse Sox2-17 chimeric transcription factor (super-Sox)
• Depositing Lab: Hans Schöler
• Publication: MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147.e9. doi: 10.1016/j.stem.2023.11.010. Epub 2023 Dec 22.

Backbone

• Vector backbone: pMXs
• Backbone size w/o insert (bp): 4627
• Total vector size (bp): 5832
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sox2-17
• Alt name: superSox
• Alt name: S*
• Species: M. musculus (mouse)
• Insert Size (bp): 1205
• Mutation: Sox2-17 is engineered highly cooperative chimeric transcription factor, where the following elements of Sox2 were swapped with Sox17: 43-47, 61, 65-86 and CTD
• Entrez Gene: Sox2 (a.k.a. Sox-2, lcc, ysb)
• Promoter: 5'LTR

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: gacggcatcgcagcttggatacac
• 3′ sequencing primer: CCCTTTTTCTGGAGACTAAATAAAATC

Resource Information

• Supplemental Documents:
  • pMXs-Sox2-17.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.09.23.509242v1 for BioRxiv preprint