pCFJ150 - pDESTttTi5605[R4-R3]
(Plasmid #19329)

• Depositing Lab: Erik Jorgensen
• Publication: Christian et al

Backbone

• Vector backbone: N/A
• Backbone size w/o insert (bp): 2354
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Unknown
• Growth instructions: ccdB Survival T1 phage resistant cells. standard conditions.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pDESTttTi5605[R4-R3]
• Species: C. elegans (nematode)
• Insert Size (bp): 6777

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 17 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Invitrogen Gateway 3-fragment compatible destination vector. Contains C.Briggsae unc-119 rescue fragment. Also contains genomic regions flanking ttTi5605 Mos1 insertion to generate MosSCI inserts at this locus.

Please note that due to a bug in the Addgene map algorithm, the attR sites are depicted as attR1 and attR2, when in fact they are attR3 and attR4. Please refer to the author's map for an accurate depiction of these sites.