pCFJ68 - Exp5605_Punc-122::GFP::unc-54utr
(Plasmid #19325)

• Depositing Lab: Erik Jorgensen
• Publication: Christian et al

Backbone

• Vector backbone: N/A
• Backbone size w/o insert (bp): 2448
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Punc-122::GFP
• Alt name: unc-122:GFP
• Alt name: ttTI5605
• Species: C. elegans (nematode)
• Insert Size (bp): 7111

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Use this plasmid as a positive control for insertion frequency by MosSCI at ttTi5605 locus. Correct insertion will give dim (not visible on dissection scope) fluorescence in the coelomycytes.