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Addgene

PB-SRT-Puro_BC21
(Plasmid #193163)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193163 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB-SRT-Puro
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Barcoded piggyBac Puro SRT
  • Species
    H. sapiens (human)
  • Mutation
    Barcode: TTGG

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-SRT-Puro_BC21 was a gift from Rob Mitra (Addgene plasmid # 193163 ; http://n2t.net/addgene:193163 ; RRID:Addgene_193163)

  • For your References section:

    Measuring transcription factor binding and gene expression using barcoded self-reporting transposon calling cards and transcriptomes. Lalli M, Yen A, Thopte U, Dong F, Moudgil A, Chen X, Milbrandt J, Dougherty JD, Mitra RD. NAR Genom Bioinform. 2022 Aug 31;4(3):lqac061. doi: 10.1093/nargab/lqac061. eCollection 2022 Sep. 10.1093/nargab/lqac061 PubMed 36062164
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