PB-SRT-Puro_BC16
(Plasmid
#193158)
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PurposeBarcoded piggyBac self-reporting transposon with puromycin marker for mammalian calling cards
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 193158 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePB-SRT-Puro
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBarcoded piggyBac Puro SRT
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SpeciesH. sapiens (human)
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MutationBarcode: CGTA
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-SRT-Puro_BC16 was a gift from Rob Mitra (Addgene plasmid # 193158 ; http://n2t.net/addgene:193158 ; RRID:Addgene_193158) -
For your References section:
Measuring transcription factor binding and gene expression using barcoded self-reporting transposon calling cards and transcriptomes. Lalli M, Yen A, Thopte U, Dong F, Moudgil A, Chen X, Milbrandt J, Dougherty JD, Mitra RD. NAR Genom Bioinform. 2022 Aug 31;4(3):lqac061. doi: 10.1093/nargab/lqac061. eCollection 2022 Sep. 10.1093/nargab/lqac061 PubMed 36062164