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Addgene

Human Bcl-2/pcDNA3
(Plasmid #19279)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19279 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human Bcl-2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Entrez Gene
    BCL2 (a.k.a. Bcl-2, PPP1R50)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human Bcl-2/pcDNA3 was a gift from Philip Leder (Addgene plasmid # 19279 ; http://n2t.net/addgene:19279 ; RRID:Addgene_19279)

  • For your References section:

    F16, a mitochondriotoxic compound, triggers apoptosis or necrosis depending on the genetic background of the target carcinoma cell. Fantin VR, Leder P. Cancer Res. 2004 Jan 1. 64(1):329-36. 10.1158/0008-5472.CAN-03-0899 PubMed 14729642
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