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Addgene

pLY017SB_pAAV-U6sg(BbsI)-EFS-Thy1.1-P2A-SB100X
(Plasmid #192151)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192151 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Lenti
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Mutation
    NA

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLY017SB_pAAV-U6sg(BbsI)-EFS-Thy1.1-P2A-SB100X was a gift from Sidi Chen (Addgene plasmid # 192151 ; http://n2t.net/addgene:192151 ; RRID:Addgene_192151)

  • For your References section:

    In vivo CRISPR screening in CD8 T cells with AAV-Sleeping Beauty hybrid vectors identifies membrane targets for improving immunotherapy for glioblastoma. Ye L, Park JJ, Dong MB, Yang Q, Chow RD, Peng L, Du Y, Guo J, Dai X, Wang G, Errami Y, Chen S. Nat Biotechnol. 2019 Nov;37(11):1302-1313. doi: 10.1038/s41587-019-0246-4. Epub 2019 Sep 23. 10.1038/s41587-019-0246-4 PubMed 31548728
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