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Addgene

Red cGull
(Plasmid #191853)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191853 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 6570
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Red cGull
  • Alt name
    Red cGMP visualizing fluorescent protein
  • Species
    Synthetic
  • Insert Size (bp)
    1170
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer BGH Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Red cGull was a gift from Takashi Tsuboi (Addgene plasmid # 191853 ; http://n2t.net/addgene:191853 ; RRID:Addgene_191853)

  • For your References section:

    Development of a red fluorescent protein-based cGMP indicator applicable for live-cell imaging. Takizawa M, Osuga Y, Ishida R, Mita M, Harada K, Ueda H, Kitaguchi T, Tsuboi T. Commun Biol. 2022 Sep 5;5(1):833. doi: 10.1038/s42003-022-03790-2. 10.1038/s42003-022-03790-2 PubMed 36064581
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