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Addgene

pBabe Angptl4 puro
(Plasmid #19156)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19156 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-puro
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Need to grow in STBL3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    angiopoietin-like 4
  • Alt name
    ANGPTL4, ANGPTL2, ARP4, FIAF, HFARP, NL2, PGAR, pp1158
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1200
  • Entrez Gene
    ANGPTL4 (a.k.a. ARP4, FIAF, HARP, HFARP, NL2, PGAR, TGQTL, UNQ171, pp1158)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    David Padua
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PCR out Angptl4 cDNA, used TOPO cloning kit and generated BglII sites at the ends. Cloned into BamHI site of pBabe which destroys BamHI site. Checked orientation with EcoRI digest.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe Angptl4 puro was a gift from Joan Massague (Addgene plasmid # 19156 ; http://n2t.net/addgene:19156 ; RRID:Addgene_19156)

  • For your References section:

    TGFbeta primes breast tumors for lung metastasis seeding through angiopoietin-like 4. Padua D, Zhang XH, Wang Q, Nadal C, Gerald WL, Gomis RR, Massague J. Cell. 2008 Apr 4. 133(1):66-77. 10.1016/j.cell.2008.01.046 PubMed 18394990
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