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Addgene

LM627
(Plasmid #191551)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191551 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29b+
  • Total vector size (bp) 5698
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    encodes E. coli lethal ccdb gene for selection, grow only in ccdb resistant strains such as NEBStable, or ccdB Survival
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ccdb
  • Species
    E. Coli
  • Insert Size (bp)
    484
  • Tag / Fusion Protein
    • SNAC-tag, HIS-tag

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LM627 was a gift from David Baker (Addgene plasmid # 191551 ; http://n2t.net/addgene:191551 ; RRID:Addgene_191551)

  • For your References section:

    Hallucinating symmetric protein assemblies. Wicky BIM, Milles LF, Courbet A, Ragotte RJ, Dauparas J, Kinfu E, Tipps S, Kibler RD, Baek M, DiMaio F, Li X, Carter L, Kang A, Nguyen H, Bera AK, Baker D. Science. 2022 Oct 7;378(6615):56-61. doi: 10.1126/science.add1964. Epub 2022 Sep 15. 10.1126/science.add1964 PubMed 36108048
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